nebnext microbiome dna enrichment kit

Marketing Communications Manager Microbiome Diversity is Retained after Enrichment with the NEBNext Microbiome DNA Enrichment Kit DNA was purified from pooled human saliva DNA (Innovative Research) and enriched using the NEBNext Microbiome DNA Enrichment Kit. You have been idle for more than 20 minutes, for your security you have been logged out. The kit is compatible with downstream applications including next . This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). Tel: 978-380-7464 Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. Learn more and request a sample! One individual reaction, from start to finish, will require 4 ml of 1X Bind/wash Buffer. Facilitates the enrichment or exclusion of microbial DNA from samples containing methylated host DNA (including human). 20% off NEBNext RNA library seq kits; SALE: 50% off Growth Factor Elisa Kits; Information; Front page; Suppliers; Campaigns; News & Focus. Brningstr. Contact your local subsidiary or distributor. The NEBNext Microbiome DNA Enrichment Kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. mitochondria, chloroplast) from eukaryote nuclear DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles. Please sign back in to continue your session. Prepare Genomic DNA DNA should be free of proteins, proteinase A, SDS and organic solvents; size should be 15 kb foroptimal performance. Add DNA to MBD2-Fc Magnetic Beads. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Catalog No. Add to Wish List . South East Asia's leading provider for life sciences kits, reagents, molecular diagnostics kits (Mdx), instruments and general laboratory equipments. Can the Microbiome DNA Enrichment Kit also be used for enrichment of organelles such as mitochondria and chloroplasts? This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. Incubate the reactionfor 15 minutes at room temperature with gentle mixing. Collect Supernatant Fraction containing enriched microbialDNA and Bead Fraction-Containing Host DNA. Importantly, microbial diversity remains intact after enrichment. (Because the HOMD collection is not comprehensive, ~80% of reads in the enriched samples do not map to either database.) New England Biolabs Reads were mapped using Bowtie 0.12.7[2] with typical settings (2 mismatches in a 28 bp seed region, etc.). Nebnext Microbiome Kit, supplied by New England Biolabs, used in various techniques. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. The NEBNext Microbiome DNA Enrichment Kit uses a simple and fast magnetic bead-based method to selectively bind and remove CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit is definitiv one Method I will try in the lab in addition to other methods. Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. Refer to the manual for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers. Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial genomic DNA from samples contain- ing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Are you doing COVID-19 related research? Erbay Yigit, David I. Hernandez, Joshua T. Trujillo, Eileen Dimalanta, and C. Donovan Bailey NC0514634. For maximum convenience and value, columns and buffers are also available separately. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Since only a small percentage of sequencing reads pertain to the microbes of interest, obtaining sufficient sequence coverage of the microbiome DNA can become cost-prohibitive or even technically infeasible. Hello, Sign in. ChIP kits & reagents; Companion products; CUT&RUN / CUT&TAG; ELISA; Item NEBNextMicrobiome DNA Enrichment Kit; Company New England Biolabs; Price Pricing Info Supplier Page View Company Product Page; Catalog Number E2612S; Quantity 6 reactions; Applications DNA Analysis; Source Bacterial; Nucleic Acid Type Genomic DNA; Temperature Multi-Temperature Rhizosphere microbiome analyses. Combine MBD2-Fc and Magnetic Beads in 1X Bind/wash Buffer. Start by following these tips for DNA quantification and assessing purity. You have been idle for more than 20 minutes, for your security you have been logged out. The NEBNext Microbiome DNA Enrichment Kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. The NEBNext Microbiome DNA Enrichment Kit makes it possible to substantially enrich samples for non-host, microbial DNA, while retaining microbial diversity, thereby improving the quality and cost-effectiveness of downstream analyses. Libraries were prepared from unenriched and enriched samples and sequenced on the SOLiD 4 platform. 50; Geb. Your profile has been mapped to an Institution, please sign back for your profile updates to be completed. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. ZERO BIAS - scores, article reviews, protocol conditions and more . These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner. This product is intended for research purposes only. The video also includes tips for optimization as well as safe stopping points. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors. Microbiome DNA Enrichment NEBNext Magnetic Separation Rack NEBNext Microbiome DNA Enrichment Kit RNA Library Prep NEBNext ARTIC SARS-CoV-2 RT-PCR Module NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads NEBNext Library Quant DNA Standards Monarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. The NEBNext Microbiome DNA Enrichment Kit enables enrichment of non-CpG-methylated DNA from samples contaminated with eukaryotic-cell DNA. Agencourt Ampure Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090), NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090), NEBNext dA-Tailing Module Protocol (E6053), NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090), NEBNext End Repair Module Protocol (E6050), NEBNext DNA Sample Prep Master Mix Set for 454 End Repair Module Protocol (E6070), NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071), NEBNext Quick Ligation Module Protocol (E6056), NEBNext DNA Sample Prep Master Mix Set for 454 Quick Ligation Module Protocol (E6070), NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090), Protocol for use with NEBNext Microbiome DNA Enrichment (E2612), Protocol for use with NEBNext DNA Library Prep Master Mix Set for Illumina (E6040), Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600). microbiome dna enrichment kit (New England Biolabs) 97. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. NEBNext suite of products continues to evolve to support next generation sequencing with sample preparation tools that streamline workflows, minimize inputs, and improve library yields and quality. Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. Cart This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Ipswich, MA 01938 Incubate the reaction for 10 minutes at room temperature. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA & RNA cleanup. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA & RNA cleanup. SKU. international site. Contact your local subsidiary or distributor. Monarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. For more information about New England Biolabs visit www.neb.com. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. 240 County Road High concordance continues even to very low abundance species (inset). The method uses the MBD2-Fc protein, which is composed of the methylated CpG-specific binding protein MBD2, fused to the Fc fragment of human IgG. New England Biolabs Releases the NEBNext Microbiome DNA Enrichment Kit, DNA Assembly, Cloning and Mutagenesis Kits, Supporting Infectious Disease Research & Development. For Help With Your Order Contact our Customer Service Team by email or call 1-800-NEB-LABS. 16 NEBNext FFPE DNA Repair Mix 17 NEBNext Microbiome DNA Enrichment Kit 18-19 NEBNext Library Quant Kit for Illumina 20-21 NEBNext Direct Genotyping Solution Ordering Information TOOLS & RESOURCES Visit NEBNext.com to find: The full list of products available Video protocols Online tutorials to help with product selection, general Please sign back in to continue your session. But unfortunately this kit didnt bring us good results in the last year. E2612S . Importantly, microbial diversity remains intact after enrichment. B852 Publications related to Microbiome DNA Enrichment, DNA Assembly, Cloning and Mutagenesis Kits, Supporting Infectious Disease Research & Development, NGS Sample Prep & Target Enrichment Products. Learn more and request a sample! Add to Cart. All Rights Reserved, Effective enrichment of microbial genomic DNA from samples contaminating host DNA, Enables microbiome whole genome sequencing, even for samples with high levels of host DNA, Compatible with downstream applications including next generation sequencing on all platforms, qPCR and end point PCR, Suitable for a wide range of sample types, Reboot your Bench practical safety tips for your laboratory, NEBNext Ultra II Kits The Core of your NGS Library Prep, NEBNext Single Cell/Low Input RNA Library Prep, RNA Depletion Get more relevant reads from your input material, NEBNext Direct Genotyping Solution for Illumina. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial genomic DNA from samples contain- ing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Microbiome samples are commonly dominated by host DNA (up to 99%). If you don't see your country above, please visit our Monarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. NEBNext Microbiome DNA Enrichment Kit To Request Technical Support Fill out our Technical Support Form , email us, or call 1-800-632-7799. ZERO BIAS - scores, article reviews, protocol conditions and more This complicates the genetic analyses of these samples, particularly total microbiome DNA sequencing studies. New England Biolabs GmbH The Fc fragment binds readily to Protein A . Classical cell culture media; . Email: martin@neb.com. A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA. You have been idle for more than 20 minutes, for your security you have been logged out. The NEBNext Solution The NEBNext Microbiome DNA Enrichment Kit addresses this problem by providing a quick and effective way to remove contaminating host DNA, thereby enriching for microbiome DNA. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next generation sequencing, qPCR and endpoint PCR. The NEBNext Microbiome DNA Enrichment Kit uses a simple and fast magnetic bead-based method to selectively bind and remove CpG-methylated host DNA. Further information can be found in our Technical Resources section or at neb.com. The graph shows percentages of 500M-537M SOLiD4 50bp reads that mapped to either the Human reference sequence (hg19) or to a microbe listed in Human Oral Microbiome Database (HOMD)[1]. Abundance is inferred from the number of reads mapping to each species as a percentage of all reads mapping to HOMD. Account & Lists Returns & Orders. Startseite > Next Generation Sequencing > NEBNext Microbiome DNA Enrichment Kit. Genome and Metagenome Sequencing: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues. (2013) The NEBNext Microbiome DNA enrichment kit technical guide shows that over 90% of human DNA can be removed from saliva samples using this kit. For Help With Your Order Contact our Customer Service Team by email or call 1800 934 218. Be the first to review this product . Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. Targeted Enrichment Next Generation Sequencing Prepare 1X Bind/wash Buffer on ice by diluting 1 part NEBNext Bind/wash Buffer (5X) with 4 parts DNase-free water. DNA was purified from pooled human saliva DNA (Innovative Research) and enriched using the NEBNext Microbiome DNA Enrichment Kit. NEBNext Microbiome DNA Enrichment Kit. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling quick and easy removal of the contaminating host DNA in about 30 minutes. Refer to the manual (NEB #E7335) for a list of NEBNext library prep kits that contain protocols for use of NEBNext adaptors and primers. Erbay describes the findings of his recent paper. Libraries were prepared from unenriched and enriched samples, followed by sequencing on the SOLiD 4 platform. international site. If you don't see your country above, please visit our The NEBNext Microbiome DNA Enrichment Kit uses a simple and fast magnetic bead-based method to selectively bind and remove CpG-methylated host DNA. The method uses the MBD2-Fc protein, which is composed of the methylated CpG-specific binding protein MBD2, fused to the Fc fragment of human IgG. * Niesseria flavescens This organism may have unusual methylation density, allowing it to bind the enriching beads at a low level. (2014) For maximum convenience and value, columns and buffers are also available separately. The graph shows a comparison between relative abundance of each bacterial species listed in HOMD[1] before and after enrichment with the NEBNext Microbiome DNA Enrichment Kit. NGS sample prep & target enrichment; Sanger sequencing reagents; Cell culturing & reagents. Are you doing COVID-19 related research? Learn more and request a sample! Please sign back in to continue your session. The microbial DNA-enriched . Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling quick and easy removal of the contaminating host DNA in about 30 minutes. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. Microbiome sample analysis is commonly confounded by the presence of host-cell DNA in the sample. NEBNext Microbiome DNA Enrichment Kit To Request Technical Support Fill out our Technical Support Form , email us, or call 1800 934 218. Importantly, microbial diversity remains intact after enrichment. Reads were mapped using Bowtie 0.12.7[2] with typical settings (2 mismatches in a 28bp seed region, etc). Qty. Established in the mid 1970's, New England Biolabs, Inc. is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. The method uses the MBD2-Fc protein, which is composed of the methylated CpG-specific binding protein MBD2, fused to the Fc fragment of human IgG. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and seven subsidiaries located in Canada, China, France, Germany, Japan, Singapore and the UK. Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. For more information about commercial rights, please email us atbusdev@neb.com. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. The NEBNext Microbiome DNA Enrichment Kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. Importantly, microbial diversity remains intact after enrichment. LabMal is Malaysia's leading provider for life sciences kits, reagents, molecular diagnostics kits (Mdx), instruments and general laboratory equipments. Genomic DNA from 0.5 g soil was extracted with the PowerLyzer PowerSoil DNA Isolation Kit (Qiagen, Germany) following the manufacturer's protocol. Novel method to enrich microbial genomic DNA for more affordable microbiome next generation DNA sequencing, Ipswich, MA (May 8, 2013) New England Biolabs (NEB) announces the release of the NEBNext Microbiome DNA Enrichment Kit, which uses a novel method to separate microbial DNA from host DNA, thereby reducing the high cost of sequencing microbiome DNA to a more practical level. For Questions Related to NEB Products and Offers Contact your local US Sales Representative . NEBNext products are available at a permanent low price that can not be further discounted. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8. Data Analysis - NEBNext Library Quant Kit (E7630), Experimental Considerations - NEBNext Library Quant Kit (E7630), NEBNext Library Quant Kit Protocol - NEBNext Library Quant Kit (E7630), NEBNext Library Quant Kit Quick Protocol (E7630), Expected Results - NEBNext Library Quant Kit (E7630), Protocol for use with NEBNext Ultra II End Repair/dA-Tailing Module (E7546), Protocol for a PCR reaction using NEBNext Ultra II Q5 Master Mix (M0544), Setting up the PCR Reactions < 96 Samples and 96 Samples - 96 Single Index Kit (E6609), Index Pooling Guidelines - 96 Single Index Kit (E6609), Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2) (E7780), Addressing Challenges in Microbiome DNA Analysis, Behind the Paper: Using the Human Methyl-Binding Domain to Partition Genomic DNA Derived from Plant Tissues, Tips for Optimizing DNA Inputs in NGS Library Construction, 12 Quick Tips for NGS Library Preparation, George R. Feehery, Erbay Yigit, Samuel O. Oyola, Bradley W. Langhorst, Victor T. Schmidt, Fiona J. Stewart, Eileen T. Dimalanta, Linda A. Amaral-Zettler, Theodore Davis, Michael A. Quail, Sriharsa Pradhan For more information, visit www.neb.com/microbiome. In one tube, add 16 l of MBD2-Fc protein and 160 l of Protein A Magnetic Beads. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. $195.11. Wash beads two times in Bind/wash Reaction Buffer. Are you doing COVID-19 related research? For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. Can the Microbiome DNA Enrichment Kit also be used to enrich for methylated host DNA rather than for microbial DNA? Advantages: e-mail: info.de@neb.com, Copyright 2022 New England Biolabs. To save your cart and view previous orders, sign in to your NEB account. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA & RNA cleanup. DNA quality and quantity were evaluated on a 1% agarose gel and with a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). Can microbiome-enrichment techniques be used to partition plant DNA? NEBNext Microbiome DNA Enrichment Kit. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling quick and easy removal of the contaminating host DNA in about 30 minutes. One individual reaction, from start to finish, will require 4 ml of 1X Bind/wash Buffer. . While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. Importantly, microbial diversity remains intact after enrichment. NEB continues to expand its product offerings into areas related to PCR, gene expression, library preparation for next generation sequencing, cellular analysis, epigenetics and RNA analysis. E2612S NEBNext Microbiome DNA Enrichment Kit Packaging Unit: 6 rxns Code NACRES: NA.55 225,00 -+ Add to Cart . The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types, including samples with high levels of contaminating host DNA, (3,4) and is also effective for separation of organelle DNA (e.g., mitochondria, chloroplast) from eukaryote nuclear DNA (5). If you don't see your country above, please visit our Download Citation | Diagnostic biologique des ostoarthrites infectieuses | Rsum Les infections osto-articulaires (IOA) sont des infections complexes et invalidantes pouvant mettre en jeu . To save your cart and view previous orders, sign in to your NEB account. DNA Modifying Enzymes and Cloning Technologies, SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit 96 rxns, Gel Loading Dye, Purple (6X), no SDS 4.0 ml, NEB, NEB PCR Cloning Kit (Without Competent Cells) 20 rxns, Luna Universal Probe One-Step RT-qPCR Kit, Magnesium Sulfate (MgSO4) Solution 6.0 ml, EnGen sgRNA Synthesis Kit, S. pyogenes 20 rxns, Effective enrichment of microbial genomic DNA from samples contaminating host DNA, Enables microbiome whole genome sequencing, even for samples with high levels of host DNA, Compatible with downstream applications including next-generation sequencing on all platforms, qPCR and endpoint PCR, Suitable for a wide range of sample types, Optional protocol to retain separated host DNA, Also effective for separation of organelle DNA (e.g. In one tube, add 16 l of MBD2-Fc protein and 160 l of Protein A Magnetic Beads. New England Biolabs nebnext microbiome dna enrichment kit Nebnext Microbiome Dna Enrichment Kit, supplied by New England Biolabs, used in various techniques. Microbiome DNA analysis can be challenging due to the high percentage of host DNA present in many samples. international site. The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Contact your local subsidiary or distributor. The kit exploits the different prevalences of CpG methylation in the genomes of microbial and eukaryotic organisms. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. Other Niesseria species (N. mucosa, N. sicca and N. elognata) are represented, but do not exhibit this anomalous enrichment. Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Information on trademarks can be found here. The kit is compatible with downstream applications including next . Some studies on respiratory samples (saliva) have shown poor results using the NEBNext Microbiome DNA enrichment kit, with an average of 90.83% human DNA still remaining post depletion (Marotz et al . Prepare 1X Bind/wash Buffer on ice by diluting 1 part NEBNext Bind/wash Buffer (5X) with 4 parts DNase-free water. DNA Assembly, Cloning and Mutagenesis Kits, Supporting Infectious Disease Research & Development, NGS Sample Prep & Target Enrichment Products. For Customers Outside of Australia Contact your local subsidiary or distributor . To save your cart and view previous orders, sign in to your NEB account. The NEBNext Microbiome DNA Enrichment Kit is suitable for a wide range of sample types, including samples with high levels of contaminating host DNA, (3,4) and is also effective for separation of organelle DNA (e.g., mitochondria, chloroplast) from eukaryote nuclear DNA (5). Bioz Stars score: 94/100, based on 1 PubMed citations. Bioz Stars score: 97/100, based on 40 PubMed citations. Get a quick overview of NGS library preparation from an NEB scientist. /PRNewswire/ -- New England Biolabs (NEB) announces the release of the NEBNext Microbiome DNA Enrichment Kit, which uses a novel method to separate microbial. Although microbiome whole genome sequencing provides more information than other methods, such as 16S analysis, this method has proven difficult for many samples, due to the presence of contaminating host genomic DNA, says Fiona Stewart, Product Marketing Manager, Next Generation Sequencing at NEB. For maximum convenience and value, columns and buffers are also available separately. Facilitates the enrichment or exclusion of microbial DNA from samples containing methylated host DNA (including human). D-65926 Frankfurt am Main, Tel: +49-69/305-23140 We compared 501M 50bp SOliD4 reads in the enriched dataset to 537M 50bp SOLiD4 reads in the unenriched dataset. Contact information: Deana D. Martin, Ph.D.

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